As of September 2021, the most populous cities in Florida, along with their estimated population at that time, are as follows:

1. Jacksonville – Approximately 926,000 residents.
2. Miami – Approximately 467,000 residents.
3. Tampa – Approximately 399,700 residents.
4. Orlando – Approximately 287,400 residents.
5. St. Petersburg – Approximately 271,300 residents.
6. Hialeah – Approximately 235,500 residents.
7. Tallahassee – Approximately 199,900 residents.
8. Fort Lauderdale – Approximately 184,400 residents.
9. Port St. Lucie – Approximately 201,700 residents.
10. Cape Coral – Approximately 195,300 residents.

Please note that these population figures are based on estimates from 2021 and are subject to change. It’s recommended to consult up-to-date sources for the most recent population data.